地區:
年份(fen):
2021
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03
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02
瀏覽(lan)次(ci)數
1225
近日，Frontiers in Plant Science 在(zai)線(xian)發(fa)表中(zhong)(zhong)國(guo)(guo)農(nong)(nong)(nong)大王建華和中(zhong)(zhong)國(guo)(guo)農(nong)(nong)(nong)科院劉允軍團隊題為“ Transcriptome Analysis of Maize Immature Embryos Reveals the Roles of Cysteine in Improving Agrobacterium Infection Efficiency”研究(jiu)論文(wen)，系統(tong)解析了半胱(guang)(guang)氨酸提(ti)(ti)高農(nong)(nong)(nong)桿菌介導(dao)的(de)(de)(de)玉米(mi)幼胚(pei)轉(zhuan)化(hua)效(xiao)率(lv)(lv)的(de)(de)(de)機理(li)。 最近幾年，玉米(mi)的(de)(de)(de)遺傳轉(zhuan)化(hua)效(xiao)率(lv)(lv)不斷提(ti)(ti)高，其中(zhong)(zhong)抗氧(yang)化(hua)劑(ji)半胱(guang)(guang)氨酸可(ke)明顯提(ti)(ti)高玉米(mi)幼胚(pei)的(de)(de)(de)農(nong)(nong)(nong)桿菌轉(zhuan)化(hua)效(xiao)率(lv)(lv)，但其機理(li)尚不明確，在(zai)本(ben)研究(jiu)中(zhong)(zhong)作者證(zheng)明通過(guo)在(zai)共培(pei)養(yang)培(pei)養(yang)基(ji)中(zhong)(zhong)添加半胱(guang)(guang)氨酸可(ke)以(yi)明顯HiII和Z31兩個(ge)品(pin)種的(de)(de)(de)農(nong)(nong)(nong)桿菌轉(zhuan)化(hua)效(xiao)率(lv)(lv)，半胱(guang)(guang)氨酸處(chu)理(li)后的(de)(de)(de)幼胚(pei)活(huo)性氧(yang)的(de)(de)(de)含(han)量(liang)要(yao)高于對照組，通過(guo)轉(zhuan)錄組測序進一步研究(jiu)證(zheng)明，半胱(guang)(guang)氨酸處(chu)理(li)后939個(ge)基(ji)因表達上(shang)調，這些基(ji)因主要(yao)參(can)與(yu)氧(yang)化(hua)還原反(fan)應過(guo)程(cheng)，另(ling)外(wai)有549個(ge)基(ji)因表達下調，主要(yao)參(can)與(yu)細胞壁和細胞膜(mo)代謝過(guo)程(cheng)。 Maize Agrobacterium-mediated transformation efficiency has been greatly improved in recent years. Antioxidants, such as, cysteine, can significantly improve maize transformation frequency through improving the Agrobacterium infection efficiency. However, the mechanism underlying ...