地區:
年份:
2021
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03
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02
瀏覽次數
1225
近日,Frontiers in Plant Science 在線發表中(zhong)國農(nong)(nong)大王(wang)建華和(he)中(zhong)國農(nong)(nong)科(ke)院劉允軍團隊(dui)題為(wei)“ Transcriptome Analysis of Maize Immature Embryos Reveals the Roles of Cysteine in Improving Agrobacterium Infection Efficiency”研究論文,系統(tong)解(jie)析了半胱(guang)(guang)氨(an)(an)酸(suan)提(ti)(ti)高(gao)(gao)農(nong)(nong)桿菌(jun)介導的(de)(de)玉米幼(you)胚轉(zhuan)(zhuan)化(hua)效率的(de)(de)機理(li)。 最近幾(ji)年,玉米的(de)(de)遺傳轉(zhuan)(zhuan)化(hua)效率不(bu)斷提(ti)(ti)高(gao)(gao),其(qi)中(zhong)抗(kang)氧(yang)化(hua)劑半胱(guang)(guang)氨(an)(an)酸(suan)可明(ming)顯提(ti)(ti)高(gao)(gao)玉米幼(you)胚的(de)(de)農(nong)(nong)桿菌(jun)轉(zhuan)(zhuan)化(hua)效率,但其(qi)機理(li)尚不(bu)明(ming)確,在本(ben)研究中(zhong)作者(zhe)證明(ming)通(tong)過在共培養培養基中(zhong)添加(jia)半胱(guang)(guang)氨(an)(an)酸(suan)可以明(ming)顯HiII和(he)Z31兩個品種的(de)(de)農(nong)(nong)桿菌(jun)轉(zhuan)(zhuan)化(hua)效率,半胱(guang)(guang)氨(an)(an)酸(suan)處理(li)后的(de)(de)幼(you)胚活性氧(yang)的(de)(de)含量要(yao)高(gao)(gao)于對照組,通(tong)過轉(zhuan)(zhuan)錄(lu)組測序進一步(bu)研究證明(ming),半胱(guang)(guang)氨(an)(an)酸(suan)處理(li)后939個基因表達(da)(da)上(shang)調,這些(xie)基因主要(yao)參(can)與(yu)氧(yang)化(hua)還(huan)原(yuan)反應過程,另外有549個基因表達(da)(da)下調,主要(yao)參(can)與(yu)細(xi)胞(bao)壁(bi)和(he)細(xi)胞(bao)膜(mo)代謝過程。 Maize Agrobacterium-mediated transformation efficiency has been greatly improved in recent years. Antioxidants, such as, cysteine, can significantly improve maize transformation frequency through improving the Agrobacterium infection efficiency. However, the mechanism underlying ...