地區:
年份:
2021
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03
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02
瀏覽(lan)次數
1225
近日,Frontiers in Plant Science 在線發(fa)表中(zhong)(zhong)國農(nong)大王建華和(he)中(zhong)(zhong)國農(nong)科院劉允軍團(tuan)隊題為“ Transcriptome Analysis of Maize Immature Embryos Reveals the Roles of Cysteine in Improving Agrobacterium Infection Efficiency”研(yan)究(jiu)論文,系統解(jie)析(xi)了半胱(guang)氨(an)酸(suan)(suan)提高(gao)農(nong)桿(gan)菌介導的(de)(de)(de)玉(yu)米幼胚轉化(hua)(hua)(hua)效(xiao)率(lv)的(de)(de)(de)機(ji)理。 最近幾年,玉(yu)米的(de)(de)(de)遺傳轉化(hua)(hua)(hua)效(xiao)率(lv)不斷提高(gao),其(qi)中(zhong)(zhong)抗氧化(hua)(hua)(hua)劑半胱(guang)氨(an)酸(suan)(suan)可(ke)明(ming)顯(xian)提高(gao)玉(yu)米幼胚的(de)(de)(de)農(nong)桿(gan)菌轉化(hua)(hua)(hua)效(xiao)率(lv),但其(qi)機(ji)理尚不明(ming)確,在本研(yan)究(jiu)中(zhong)(zhong)作者證(zheng)明(ming)通過(guo)在共培(pei)養培(pei)養基中(zhong)(zhong)添加半胱(guang)氨(an)酸(suan)(suan)可(ke)以明(ming)顯(xian)HiII和(he)Z31兩個(ge)品種的(de)(de)(de)農(nong)桿(gan)菌轉化(hua)(hua)(hua)效(xiao)率(lv),半胱(guang)氨(an)酸(suan)(suan)處理后(hou)的(de)(de)(de)幼胚活性氧的(de)(de)(de)含(han)量要(yao)(yao)高(gao)于對照組,通過(guo)轉錄(lu)組測(ce)序進一步(bu)研(yan)究(jiu)證(zheng)明(ming),半胱(guang)氨(an)酸(suan)(suan)處理后(hou)939個(ge)基因表達上調(diao),這些基因主要(yao)(yao)參與氧化(hua)(hua)(hua)還原反應過(guo)程,另(ling)外(wai)有549個(ge)基因表達下調(diao),主要(yao)(yao)參與細(xi)胞(bao)壁和(he)細(xi)胞(bao)膜代謝過(guo)程。 Maize Agrobacterium-mediated transformation efficiency has been greatly improved in recent years. Antioxidants, such as, cysteine, can significantly improve maize transformation frequency through improving the Agrobacterium infection efficiency. However, the mechanism underlying ...