地區:
年份:
2021
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03
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02
瀏覽(lan)次數
1225
近日,Frontiers in Plant Science 在(zai)線(xian)發表中(zhong)(zhong)國農(nong)(nong)大王建華和(he)中(zhong)(zhong)國農(nong)(nong)科院劉(liu)允(yun)軍團隊(dui)題為(wei)“ Transcriptome Analysis of Maize Immature Embryos Reveals the Roles of Cysteine in Improving Agrobacterium Infection Efficiency”研(yan)(yan)究(jiu)(jiu)論文,系統解析了半(ban)(ban)胱(guang)氨酸(suan)(suan)(suan)提高農(nong)(nong)桿(gan)菌介導的玉米(mi)幼(you)胚轉(zhuan)化(hua)(hua)效(xiao)(xiao)率(lv)(lv)(lv)的機理。 最近幾年,玉米(mi)的遺傳轉(zhuan)化(hua)(hua)效(xiao)(xiao)率(lv)(lv)(lv)不斷提高,其中(zhong)(zhong)抗氧(yang)化(hua)(hua)劑半(ban)(ban)胱(guang)氨酸(suan)(suan)(suan)可明(ming)(ming)顯提高玉米(mi)幼(you)胚的農(nong)(nong)桿(gan)菌轉(zhuan)化(hua)(hua)效(xiao)(xiao)率(lv)(lv)(lv),但其機理尚不明(ming)(ming)確,在(zai)本研(yan)(yan)究(jiu)(jiu)中(zhong)(zhong)作者證明(ming)(ming)通過在(zai)共培(pei)養(yang)培(pei)養(yang)基(ji)中(zhong)(zhong)添加半(ban)(ban)胱(guang)氨酸(suan)(suan)(suan)可以明(ming)(ming)顯HiII和(he)Z31兩個品種(zhong)的農(nong)(nong)桿(gan)菌轉(zhuan)化(hua)(hua)效(xiao)(xiao)率(lv)(lv)(lv),半(ban)(ban)胱(guang)氨酸(suan)(suan)(suan)處(chu)理后(hou)的幼(you)胚活性(xing)氧(yang)的含量要(yao)高于對照(zhao)組,通過轉(zhuan)錄組測序進一步(bu)研(yan)(yan)究(jiu)(jiu)證明(ming)(ming),半(ban)(ban)胱(guang)氨酸(suan)(suan)(suan)處(chu)理后(hou)939個基(ji)因(yin)表達上調,這些基(ji)因(yin)主(zhu)(zhu)要(yao)參(can)與(yu)氧(yang)化(hua)(hua)還原反(fan)應過程,另外有549個基(ji)因(yin)表達下調,主(zhu)(zhu)要(yao)參(can)與(yu)細胞(bao)壁和(he)細胞(bao)膜代謝過程。 Maize Agrobacterium-mediated transformation efficiency has been greatly improved in recent years. Antioxidants, such as, cysteine, can significantly improve maize transformation frequency through improving the Agrobacterium infection efficiency. However, the mechanism underlying ...