近(jin)日,Frontiers in Plant Science 在線發表(biao)中(zhong)(zhong)國(guo)農大王建華和中(zhong)(zhong)國(guo)農科院劉(liu)允軍(jun)團隊(dui)題為“ Transcriptome Analysis of Maize Immature Embryos Reveals the Roles of Cysteine in Improving Agrobacterium Infection Efficiency”研究論文,系(xi)統解(jie)析了半胱氨酸(suan)提(ti)高(gao)(gao)農桿菌(jun)介導的(de)玉米(mi)幼胚(pei)轉(zhuan)(zhuan)化(hua)(hua)(hua)效率(lv)的(de)機理(li)。 最(zui)近(jin)幾年,玉米(mi)的(de)遺(yi)傳轉(zhuan)(zhuan)化(hua)(hua)(hua)效率(lv)不斷提(ti)高(gao)(gao),其中(zhong)(zhong)抗氧化(hua)(hua)(hua)劑半胱氨酸(suan)可(ke)明(ming)顯(xian)提(ti)高(gao)(gao)玉米(mi)幼胚(pei)的(de)農桿菌(jun)轉(zhuan)(zhuan)化(hua)(hua)(hua)效率(lv),但(dan)其機理(li)尚不明(ming)確,在本(ben)研究中(zhong)(zhong)作者證(zheng)明(ming)通過(guo)(guo)在共培(pei)養(yang)培(pei)養(yang)基中(zhong)(zhong)添加半胱氨酸(suan)可(ke)以明(ming)顯(xian)HiII和Z31兩個(ge)品種的(de)農桿菌(jun)轉(zhuan)(zhuan)化(hua)(hua)(hua)效率(lv),半胱氨酸(suan)處理(li)后的(de)幼胚(pei)活(huo)性氧的(de)含量要(yao)高(gao)(gao)于(yu)對照組(zu),通過(guo)(guo)轉(zhuan)(zhuan)錄(lu)組(zu)測序(xu)進一步研究證(zheng)明(ming),半胱氨酸(suan)處理(li)后939個(ge)基因(yin)表(biao)達(da)上調,這些(xie)基因(yin)主(zhu)要(yao)參(can)(can)與氧化(hua)(hua)(hua)還原反應過(guo)(guo)程(cheng),另外有549個(ge)基因(yin)表(biao)達(da)下調,主(zhu)要(yao)參(can)(can)與細胞壁和細胞膜代謝過(guo)(guo)程(cheng)。 Maize Agrobacterium-mediated transformation efficiency has been greatly improved in recent years. Antioxidants, such as, cysteine, can significantly improve maize transformation frequency through improving the Agrobacterium infection efficiency. However, the mechanism underlying ...
The free-living flatworm, Macrostomum lignano has an impressive regenerative capacity. Following injury, it can regenerate almost an entirely new organism because of the presence of an abundant somatic stem cell population, the neoblasts. This set of unique properties makes many flatworms attractive...
An overview of our assembly strategy is shown in Figure 1. We started with a SOAPdenovo- based de novo assembly of Illumina sequence data from human HapMap sample NA12878, which had a contig N50 of 11.1 kb and a scaffold N50 of 590 kb after filtering for scaffolds that were at least 3 kb in length (...
Transposable elements and expression of retrotransposons Long terminal repeat (LTR) retrotransposons were identified using structural criteria10,11. About 44% of the assembly was accounted for by TEs (Supplementary Table 8 ). LTR retrotransposons were the most abundant of these elements, repres...
Winter dormancy is a well known mechanism adopted by temperate plants, to mitigate the chilling temperature of winters. However, acquisition of sufficient chilling during winter dor-mancy ensures the normal phenological traits in subsequent growing period. Thus, low tem-perature appears to play cruc...
Whole genome protein families were classified by InterProScan analysis (Jones et al., 2014). The peptidase families were identified by BLASTP searching against MEROPS peptidase database release 9.12 with a cuto? e-value of 1e-20 (Rawlings et al., 2014). The transporters were classified on the b...