近日,Frontiers in Plant Science 在線發表(biao)中國(guo)農大(da)王(wang)建華和(he)(he)中國(guo)農科院劉允軍團隊(dui)題為“ Transcriptome Analysis of Maize Immature Embryos Reveals the Roles of Cysteine in Improving Agrobacterium Infection Efficiency”研究論文,系統解析了半(ban)(ban)(ban)胱(guang)(guang)(guang)氨(an)酸(suan)提(ti)高(gao)農桿菌(jun)介導的(de)(de)(de)玉(yu)(yu)米(mi)幼胚(pei)(pei)轉化(hua)(hua)效率的(de)(de)(de)機理。 最近幾年,玉(yu)(yu)米(mi)的(de)(de)(de)遺(yi)傳轉化(hua)(hua)效率不斷提(ti)高(gao),其中抗氧(yang)化(hua)(hua)劑半(ban)(ban)(ban)胱(guang)(guang)(guang)氨(an)酸(suan)可(ke)明顯提(ti)高(gao)玉(yu)(yu)米(mi)幼胚(pei)(pei)的(de)(de)(de)農桿菌(jun)轉化(hua)(hua)效率,但其機理尚不明確,在本研究中作者證(zheng)明通過(guo)在共培養(yang)培養(yang)基中添(tian)加(jia)半(ban)(ban)(ban)胱(guang)(guang)(guang)氨(an)酸(suan)可(ke)以明顯HiII和(he)(he)Z31兩個(ge)(ge)品種的(de)(de)(de)農桿菌(jun)轉化(hua)(hua)效率,半(ban)(ban)(ban)胱(guang)(guang)(guang)氨(an)酸(suan)處理后的(de)(de)(de)幼胚(pei)(pei)活(huo)性氧(yang)的(de)(de)(de)含量要高(gao)于對照組(zu),通過(guo)轉錄組(zu)測序(xu)進一步研究證(zheng)明,半(ban)(ban)(ban)胱(guang)(guang)(guang)氨(an)酸(suan)處理后939個(ge)(ge)基因表(biao)達上(shang)調,這些基因主要參與(yu)氧(yang)化(hua)(hua)還(huan)原(yuan)反(fan)應過(guo)程,另外有(you)549個(ge)(ge)基因表(biao)達下調,主要參與(yu)細(xi)胞壁和(he)(he)細(xi)胞膜代謝過(guo)程。 Maize Agrobacterium-mediated transformation efficiency has been greatly improved in recent years. Antioxidants, such as, cysteine, can significantly improve maize transformation frequency through improving the Agrobacterium infection efficiency. However, the mechanism underlying ...
The free-living flatworm, Macrostomum lignano has an impressive regenerative capacity. Following injury, it can regenerate almost an entirely new organism because of the presence of an abundant somatic stem cell population, the neoblasts. This set of unique properties makes many flatworms attractive...
An overview of our assembly strategy is shown in Figure 1. We started with a SOAPdenovo- based de novo assembly of Illumina sequence data from human HapMap sample NA12878, which had a contig N50 of 11.1 kb and a scaffold N50 of 590 kb after filtering for scaffolds that were at least 3 kb in length (...
Transposable elements and expression of retrotransposons Long terminal repeat (LTR) retrotransposons were identified using structural criteria10,11. About 44% of the assembly was accounted for by TEs (Supplementary Table 8 ). LTR retrotransposons were the most abundant of these elements, repres...
Winter dormancy is a well known mechanism adopted by temperate plants, to mitigate the chilling temperature of winters. However, acquisition of sufficient chilling during winter dor-mancy ensures the normal phenological traits in subsequent growing period. Thus, low tem-perature appears to play cruc...
Whole genome protein families were classified by InterProScan analysis (Jones et al., 2014). The peptidase families were identified by BLASTP searching against MEROPS peptidase database release 9.12 with a cuto? e-value of 1e-20 (Rawlings et al., 2014). The transporters were classified on the b...