近日,Frontiers in Plant Science 在線發表(biao)中(zhong)國農大王(wang)建華和中(zhong)國農科(ke)院劉允(yun)軍團(tuan)隊題為“ Transcriptome Analysis of Maize Immature Embryos Reveals the Roles of Cysteine in Improving Agrobacterium Infection Efficiency”研究論文,系統解析(xi)了半(ban)胱(guang)氨(an)酸(suan)提(ti)高農桿菌介導的(de)玉米幼(you)胚(pei)轉(zhuan)(zhuan)化(hua)效率(lv)(lv)的(de)機(ji)理(li)。 最近幾(ji)年,玉米的(de)遺(yi)傳轉(zhuan)(zhuan)化(hua)效率(lv)(lv)不斷提(ti)高,其(qi)中(zhong)抗氧(yang)化(hua)劑半(ban)胱(guang)氨(an)酸(suan)可明(ming)(ming)顯提(ti)高玉米幼(you)胚(pei)的(de)農桿菌轉(zhuan)(zhuan)化(hua)效率(lv)(lv),但(dan)其(qi)機(ji)理(li)尚不明(ming)(ming)確,在本研究中(zhong)作者證明(ming)(ming)通(tong)過在共培養培養基中(zhong)添加半(ban)胱(guang)氨(an)酸(suan)可以明(ming)(ming)顯HiII和Z31兩個品種的(de)農桿菌轉(zhuan)(zhuan)化(hua)效率(lv)(lv),半(ban)胱(guang)氨(an)酸(suan)處(chu)理(li)后的(de)幼(you)胚(pei)活性(xing)氧(yang)的(de)含(han)量要(yao)(yao)高于對照組,通(tong)過轉(zhuan)(zhuan)錄(lu)組測序進一步研究證明(ming)(ming),半(ban)胱(guang)氨(an)酸(suan)處(chu)理(li)后939個基因表(biao)達上調,這些(xie)基因主要(yao)(yao)參與氧(yang)化(hua)還(huan)原(yuan)反應過程,另(ling)外有549個基因表(biao)達下(xia)調,主要(yao)(yao)參與細胞壁和細胞膜(mo)代謝過程。 Maize Agrobacterium-mediated transformation efficiency has been greatly improved in recent years. Antioxidants, such as, cysteine, can significantly improve maize transformation frequency through improving the Agrobacterium infection efficiency. However, the mechanism underlying ...
The free-living flatworm, Macrostomum lignano has an impressive regenerative capacity. Following injury, it can regenerate almost an entirely new organism because of the presence of an abundant somatic stem cell population, the neoblasts. This set of unique properties makes many flatworms attractive...
An overview of our assembly strategy is shown in Figure 1. We started with a SOAPdenovo- based de novo assembly of Illumina sequence data from human HapMap sample NA12878, which had a contig N50 of 11.1 kb and a scaffold N50 of 590 kb after filtering for scaffolds that were at least 3 kb in length (...
Transposable elements and expression of retrotransposons Long terminal repeat (LTR) retrotransposons were identified using structural criteria10,11. About 44% of the assembly was accounted for by TEs (Supplementary Table 8 ). LTR retrotransposons were the most abundant of these elements, repres...
Winter dormancy is a well known mechanism adopted by temperate plants, to mitigate the chilling temperature of winters. However, acquisition of sufficient chilling during winter dor-mancy ensures the normal phenological traits in subsequent growing period. Thus, low tem-perature appears to play cruc...
Whole genome protein families were classified by InterProScan analysis (Jones et al., 2014). The peptidase families were identified by BLASTP searching against MEROPS peptidase database release 9.12 with a cuto? e-value of 1e-20 (Rawlings et al., 2014). The transporters were classified on the b...