近(jin)(jin)日,Frontiers in Plant Science 在線發(fa)表(biao)中國農(nong)大王建華和中國農(nong)科院劉允軍團隊題為“ Transcriptome Analysis of Maize Immature Embryos Reveals the Roles of Cysteine in Improving Agrobacterium Infection Efficiency”研究(jiu)論文,系統解析了(le)半(ban)胱(guang)氨(an)酸(suan)提高(gao)農(nong)桿菌介導的(de)(de)(de)玉米(mi)(mi)幼(you)(you)(you)胚轉(zhuan)(zhuan)化效(xiao)率的(de)(de)(de)機理(li)。 最近(jin)(jin)幾年(nian),玉米(mi)(mi)的(de)(de)(de)遺傳(chuan)轉(zhuan)(zhuan)化效(xiao)率不(bu)斷提高(gao),其中抗(kang)氧(yang)(yang)化劑半(ban)胱(guang)氨(an)酸(suan)可明顯(xian)提高(gao)玉米(mi)(mi)幼(you)(you)(you)胚的(de)(de)(de)農(nong)桿菌轉(zhuan)(zhuan)化效(xiao)率,但其機理(li)尚不(bu)明確,在本研究(jiu)中作者(zhe)證明通過(guo)在共培養培養基(ji)中添加半(ban)胱(guang)氨(an)酸(suan)可以明顯(xian)HiII和Z31兩(liang)個品種的(de)(de)(de)農(nong)桿菌轉(zhuan)(zhuan)化效(xiao)率,半(ban)胱(guang)氨(an)酸(suan)處理(li)后的(de)(de)(de)幼(you)(you)(you)胚活性氧(yang)(yang)的(de)(de)(de)含(han)量要高(gao)于對照組,通過(guo)轉(zhuan)(zhuan)錄(lu)組測序(xu)進(jin)一步研究(jiu)證明,半(ban)胱(guang)氨(an)酸(suan)處理(li)后939個基(ji)因表(biao)達上調(diao),這些(xie)基(ji)因主要參(can)與氧(yang)(yang)化還(huan)原反應過(guo)程(cheng),另外有(you)549個基(ji)因表(biao)達下調(diao),主要參(can)與細胞壁(bi)和細胞膜代謝過(guo)程(cheng)。 Maize Agrobacterium-mediated transformation efficiency has been greatly improved in recent years. Antioxidants, such as, cysteine, can significantly improve maize transformation frequency through improving the Agrobacterium infection efficiency. However, the mechanism underlying ...
The free-living flatworm, Macrostomum lignano has an impressive regenerative capacity. Following injury, it can regenerate almost an entirely new organism because of the presence of an abundant somatic stem cell population, the neoblasts. This set of unique properties makes many flatworms attractive...
An overview of our assembly strategy is shown in Figure 1. We started with a SOAPdenovo- based de novo assembly of Illumina sequence data from human HapMap sample NA12878, which had a contig N50 of 11.1 kb and a scaffold N50 of 590 kb after filtering for scaffolds that were at least 3 kb in length (...
Transposable elements and expression of retrotransposons Long terminal repeat (LTR) retrotransposons were identified using structural criteria10,11. About 44% of the assembly was accounted for by TEs (Supplementary Table 8 ). LTR retrotransposons were the most abundant of these elements, repres...
Winter dormancy is a well known mechanism adopted by temperate plants, to mitigate the chilling temperature of winters. However, acquisition of sufficient chilling during winter dor-mancy ensures the normal phenological traits in subsequent growing period. Thus, low tem-perature appears to play cruc...
Whole genome protein families were classified by InterProScan analysis (Jones et al., 2014). The peptidase families were identified by BLASTP searching against MEROPS peptidase database release 9.12 with a cuto? e-value of 1e-20 (Rawlings et al., 2014). The transporters were classified on the b...